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pha l  (Vector Laboratories)


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    Structured Review

    Vector Laboratories pha l
    Pha L, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+igg+anti+pha+l+antibody/Goat+IgG+(Control+Antibody)/pm22575585-45-11-16
    Average 96 stars, based on 557 article reviews
    pha l - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: TrkB-Like Immunoreactivity Is Present on Geniculocortical Afferents in Layer IV of Kitten Primary Visual Cortex
    Article Snippet: The blocking solution for a minority of sections contained 20 mM potassium PBS (KPBS, pH 7.4), 2.5% BSA, 0.5% Triton X-100, 3% normal horse serum (Vector, Burlingame, CA), and 0.05% thimerosal. .. After a 1-hour incubation at room temperature, sections were transferred to blocking solution containing goat IgG anti-PHA-L antibody (Vector; dilution of 1:500) and one of the following primary antibodies ( ): rabbit IgG anti-TrkB23 ( Yan et al., 1994 ; 6.2 μg/ml), rabbit IgG anti-TrkB146 ( Cabelli et al., 1996 ; 5.5 μg/ml), rabbit IgG anti-TrkB348 ( McCarty and Feinstein, 1998 ; 6.5 μg/ml), rabbit IgG anti-TrkB606 ( Costantini et al., 1999 ; 6.7 μg/ml), rabbit IgG RTB ( Huang et al., 1999a ; provided by Dr. Louis Reichardt; dilution of 1:100), or mouse IgG monoclonal anti-GAD65 ( Chang and Gottlieb, 1988 ; dilution of 1:5). ..

    Blocking Assay:

    Article Title: TrkB-Like Immunoreactivity Is Present on Geniculocortical Afferents in Layer IV of Kitten Primary Visual Cortex
    Article Snippet: The blocking solution for a minority of sections contained 20 mM potassium PBS (KPBS, pH 7.4), 2.5% BSA, 0.5% Triton X-100, 3% normal horse serum (Vector, Burlingame, CA), and 0.05% thimerosal. .. After a 1-hour incubation at room temperature, sections were transferred to blocking solution containing goat IgG anti-PHA-L antibody (Vector; dilution of 1:500) and one of the following primary antibodies ( ): rabbit IgG anti-TrkB23 ( Yan et al., 1994 ; 6.2 μg/ml), rabbit IgG anti-TrkB146 ( Cabelli et al., 1996 ; 5.5 μg/ml), rabbit IgG anti-TrkB348 ( McCarty and Feinstein, 1998 ; 6.5 μg/ml), rabbit IgG anti-TrkB606 ( Costantini et al., 1999 ; 6.7 μg/ml), rabbit IgG RTB ( Huang et al., 1999a ; provided by Dr. Louis Reichardt; dilution of 1:100), or mouse IgG monoclonal anti-GAD65 ( Chang and Gottlieb, 1988 ; dilution of 1:5). ..

    Article Title: Synaptic Density in Geniculocortical Afferents Remains Constant after Monocular Deprivation in the Cat
    Article Snippet: The blocking solution was as described above except that 3% horse serum (Vector) was added. .. Sections were blocked for 1 hr at room temperature and then transferred to blocking solution containing goat IgG anti-Pha-L antibody (Vector; dilution of 1:500) and one of the following primary antibodies: mouse IgG monoclonal anti-synaptophysin (Boehringer Mannheim, Indianapolis, IN; dilution of 1:10), mouse IgG monoclonal anti-synaptotagmin antibody (Matthew et al. 1981) (provid- ed by Dr. Louis Reichardt, University of California, San Francisco; dilution of 1:1000), mouse IgG monoclonal anti-SV2 antibody (Buckley and Kelly, 1985) (provided by Dr. Regis Kelly, University of California, San Francisco; dilution of 1:50), or mouse IgG monoclonal GAD65 (Chang and Gottlieb, 1988) (dilution of 1:5). .. The anti-GAD65 antibodies in a GAD-6 hybridoma supernatant were obtained from the Developmental Studies Hybridoma Bank maintained by the Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, and the Department of Biological Sciences, University of Iowa, Iowa City, IA, under contract N01-HD-6-2915 from the National Institute of Child Health and Human Development.

    Article Title: Synaptic Density in Geniculocortical Afferents Remains Constant after Monocular Deprivation in the Cat
    Article Snippet: The blocking solution was as described above except that 3% horse serum (Vector) was added. .. Sections were blocked for 1 hr at room temperature and then transferred to blocking solution containing goat IgG anti-Pha-L antibody (Vector; dilution of 1:500) and one of the following primary antibodies: mouse IgG monoclonal anti-synaptophysin (Boehringer Mannheim, Indianapolis, IN; dilution of 1:10), mouse IgG monoclonal anti-synaptotagmin antibody ( Matthew et al. 1981 ) (provided by Dr. Louis Reichardt, University of California, San Francisco; dilution of 1:1000), mouse IgG monoclonal anti-SV2 antibody ( Buckley and Kelly, 1985 ) (provided by Dr. Regis Kelly, University of California, San Francisco; dilution of 1:50), or mouse IgG monoclonal GAD65 ( Chang and Gottlieb, 1988 ) (dilution of 1:5). .. The anti-GAD65 antibodies in a GAD-6 hybridoma supernatant were obtained from the Developmental Studies Hybridoma Bank maintained by the Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, and the Department of Biological Sciences, University of Iowa, Iowa City, IA, under contract N01-HD-6-2915 from the National Institute of Child Health and Human Development.



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